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intense fluorescent overlap  (Oxford Instruments)


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    Oxford Instruments intense fluorescent overlap
    ( A ) Schematic representation of the experimental approach used to test whether senescent cell–derived small extracellular vesicles and particles (EVPs) can be taken up by proliferating cells. Proliferating IMR90 cells were treated with PKH67, a green <t>fluorescent</t> lipophilic membrane dye, alone or with PKH67-labeled EVPs isolated from senescent cells. ( B ) Representative immunofluorescence images of proliferating cells treated with or without PKH67-labeled sEVP fractions from senescent cells, using different sEVP volumes while keeping the PKH67 lipid dye concentration unchanged. Boxes indicate expanded region and arrows indicate PKH67 puncta in recipient cells. ( C ) Quantification of internalized PKH67-labeled sEVPs by proliferating cells. Data are presented as mean ± SD (n=3 independent experiments). Statistical significance was determined using two-sided one-way ANOVA: *p=0.023, **p=0.0010, ****p<0.0001. ( D ) Schematic representation of the experimental setup: proliferating IMR90 cells were treated with no sEVPs or sEVPs secreted by proliferating cells treated with DMSO, or senescent cells treated with the inhibitor of CCF formation, MDM2 inhibitor RG7388 (MDM2i) or DMSO vehicle. ( E ) Quantification of cGAMP concentration in proliferating cells treated with no sEVPs or sEVPs (40 μL) from proliferating cells treated with DMSO, or senescent cells treated with MDM2i or DMSO vehicle. sEVPs were isolated from equivalent numbers of cells for each condition and resuspended in identical buffer volumes. Data are presented as mean ± SD (n = 3 independent experiments). Statistical significance was determined using two-sided one-way ANOVA: *p=0.011, **p=0.0094.
    Intense Fluorescent Overlap, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 41025 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/intense fluorescent overlap/product/Oxford Instruments
    Average 99 stars, based on 41025 article reviews
    intense fluorescent overlap - by Bioz Stars, 2026-02
    99/100 stars

    Images

    1) Product Images from "Senescent cells secrete chromatin components via senescence-associated extracellular particles"

    Article Title: Senescent cells secrete chromatin components via senescence-associated extracellular particles

    Journal: bioRxiv

    doi: 10.64898/2025.12.12.694055

    ( A ) Schematic representation of the experimental approach used to test whether senescent cell–derived small extracellular vesicles and particles (EVPs) can be taken up by proliferating cells. Proliferating IMR90 cells were treated with PKH67, a green fluorescent lipophilic membrane dye, alone or with PKH67-labeled EVPs isolated from senescent cells. ( B ) Representative immunofluorescence images of proliferating cells treated with or without PKH67-labeled sEVP fractions from senescent cells, using different sEVP volumes while keeping the PKH67 lipid dye concentration unchanged. Boxes indicate expanded region and arrows indicate PKH67 puncta in recipient cells. ( C ) Quantification of internalized PKH67-labeled sEVPs by proliferating cells. Data are presented as mean ± SD (n=3 independent experiments). Statistical significance was determined using two-sided one-way ANOVA: *p=0.023, **p=0.0010, ****p<0.0001. ( D ) Schematic representation of the experimental setup: proliferating IMR90 cells were treated with no sEVPs or sEVPs secreted by proliferating cells treated with DMSO, or senescent cells treated with the inhibitor of CCF formation, MDM2 inhibitor RG7388 (MDM2i) or DMSO vehicle. ( E ) Quantification of cGAMP concentration in proliferating cells treated with no sEVPs or sEVPs (40 μL) from proliferating cells treated with DMSO, or senescent cells treated with MDM2i or DMSO vehicle. sEVPs were isolated from equivalent numbers of cells for each condition and resuspended in identical buffer volumes. Data are presented as mean ± SD (n = 3 independent experiments). Statistical significance was determined using two-sided one-way ANOVA: *p=0.011, **p=0.0094.
    Figure Legend Snippet: ( A ) Schematic representation of the experimental approach used to test whether senescent cell–derived small extracellular vesicles and particles (EVPs) can be taken up by proliferating cells. Proliferating IMR90 cells were treated with PKH67, a green fluorescent lipophilic membrane dye, alone or with PKH67-labeled EVPs isolated from senescent cells. ( B ) Representative immunofluorescence images of proliferating cells treated with or without PKH67-labeled sEVP fractions from senescent cells, using different sEVP volumes while keeping the PKH67 lipid dye concentration unchanged. Boxes indicate expanded region and arrows indicate PKH67 puncta in recipient cells. ( C ) Quantification of internalized PKH67-labeled sEVPs by proliferating cells. Data are presented as mean ± SD (n=3 independent experiments). Statistical significance was determined using two-sided one-way ANOVA: *p=0.023, **p=0.0010, ****p<0.0001. ( D ) Schematic representation of the experimental setup: proliferating IMR90 cells were treated with no sEVPs or sEVPs secreted by proliferating cells treated with DMSO, or senescent cells treated with the inhibitor of CCF formation, MDM2 inhibitor RG7388 (MDM2i) or DMSO vehicle. ( E ) Quantification of cGAMP concentration in proliferating cells treated with no sEVPs or sEVPs (40 μL) from proliferating cells treated with DMSO, or senescent cells treated with MDM2i or DMSO vehicle. sEVPs were isolated from equivalent numbers of cells for each condition and resuspended in identical buffer volumes. Data are presented as mean ± SD (n = 3 independent experiments). Statistical significance was determined using two-sided one-way ANOVA: *p=0.011, **p=0.0094.

    Techniques Used: Derivative Assay, Membrane, Labeling, Isolation, Immunofluorescence, Concentration Assay



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    intense fluorescent overlap  (Oxford Instruments)
    99
    Oxford Instruments intense fluorescent overlap
    ( A ) Schematic representation of the experimental approach used to test whether senescent cell–derived small extracellular vesicles and particles (EVPs) can be taken up by proliferating cells. Proliferating IMR90 cells were treated with PKH67, a green <t>fluorescent</t> lipophilic membrane dye, alone or with PKH67-labeled EVPs isolated from senescent cells. ( B ) Representative immunofluorescence images of proliferating cells treated with or without PKH67-labeled sEVP fractions from senescent cells, using different sEVP volumes while keeping the PKH67 lipid dye concentration unchanged. Boxes indicate expanded region and arrows indicate PKH67 puncta in recipient cells. ( C ) Quantification of internalized PKH67-labeled sEVPs by proliferating cells. Data are presented as mean ± SD (n=3 independent experiments). Statistical significance was determined using two-sided one-way ANOVA: *p=0.023, **p=0.0010, ****p<0.0001. ( D ) Schematic representation of the experimental setup: proliferating IMR90 cells were treated with no sEVPs or sEVPs secreted by proliferating cells treated with DMSO, or senescent cells treated with the inhibitor of CCF formation, MDM2 inhibitor RG7388 (MDM2i) or DMSO vehicle. ( E ) Quantification of cGAMP concentration in proliferating cells treated with no sEVPs or sEVPs (40 μL) from proliferating cells treated with DMSO, or senescent cells treated with MDM2i or DMSO vehicle. sEVPs were isolated from equivalent numbers of cells for each condition and resuspended in identical buffer volumes. Data are presented as mean ± SD (n = 3 independent experiments). Statistical significance was determined using two-sided one-way ANOVA: *p=0.011, **p=0.0094.
    Intense Fluorescent Overlap, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/intense fluorescent overlap/product/Oxford Instruments
    Average 99 stars, based on 1 article reviews
    intense fluorescent overlap - by Bioz Stars, 2026-02
    99/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Schematic representation of the experimental approach used to test whether senescent cell–derived small extracellular vesicles and particles (EVPs) can be taken up by proliferating cells. Proliferating IMR90 cells were treated with PKH67, a green fluorescent lipophilic membrane dye, alone or with PKH67-labeled EVPs isolated from senescent cells. ( B ) Representative immunofluorescence images of proliferating cells treated with or without PKH67-labeled sEVP fractions from senescent cells, using different sEVP volumes while keeping the PKH67 lipid dye concentration unchanged. Boxes indicate expanded region and arrows indicate PKH67 puncta in recipient cells. ( C ) Quantification of internalized PKH67-labeled sEVPs by proliferating cells. Data are presented as mean ± SD (n=3 independent experiments). Statistical significance was determined using two-sided one-way ANOVA: *p=0.023, **p=0.0010, ****p<0.0001. ( D ) Schematic representation of the experimental setup: proliferating IMR90 cells were treated with no sEVPs or sEVPs secreted by proliferating cells treated with DMSO, or senescent cells treated with the inhibitor of CCF formation, MDM2 inhibitor RG7388 (MDM2i) or DMSO vehicle. ( E ) Quantification of cGAMP concentration in proliferating cells treated with no sEVPs or sEVPs (40 μL) from proliferating cells treated with DMSO, or senescent cells treated with MDM2i or DMSO vehicle. sEVPs were isolated from equivalent numbers of cells for each condition and resuspended in identical buffer volumes. Data are presented as mean ± SD (n = 3 independent experiments). Statistical significance was determined using two-sided one-way ANOVA: *p=0.011, **p=0.0094.

    Journal: bioRxiv

    Article Title: Senescent cells secrete chromatin components via senescence-associated extracellular particles

    doi: 10.64898/2025.12.12.694055

    Figure Lengend Snippet: ( A ) Schematic representation of the experimental approach used to test whether senescent cell–derived small extracellular vesicles and particles (EVPs) can be taken up by proliferating cells. Proliferating IMR90 cells were treated with PKH67, a green fluorescent lipophilic membrane dye, alone or with PKH67-labeled EVPs isolated from senescent cells. ( B ) Representative immunofluorescence images of proliferating cells treated with or without PKH67-labeled sEVP fractions from senescent cells, using different sEVP volumes while keeping the PKH67 lipid dye concentration unchanged. Boxes indicate expanded region and arrows indicate PKH67 puncta in recipient cells. ( C ) Quantification of internalized PKH67-labeled sEVPs by proliferating cells. Data are presented as mean ± SD (n=3 independent experiments). Statistical significance was determined using two-sided one-way ANOVA: *p=0.023, **p=0.0010, ****p<0.0001. ( D ) Schematic representation of the experimental setup: proliferating IMR90 cells were treated with no sEVPs or sEVPs secreted by proliferating cells treated with DMSO, or senescent cells treated with the inhibitor of CCF formation, MDM2 inhibitor RG7388 (MDM2i) or DMSO vehicle. ( E ) Quantification of cGAMP concentration in proliferating cells treated with no sEVPs or sEVPs (40 μL) from proliferating cells treated with DMSO, or senescent cells treated with MDM2i or DMSO vehicle. sEVPs were isolated from equivalent numbers of cells for each condition and resuspended in identical buffer volumes. Data are presented as mean ± SD (n = 3 independent experiments). Statistical significance was determined using two-sided one-way ANOVA: *p=0.011, **p=0.0094.

    Article Snippet: The same channel thresholds were maintained for all cells, and the most intense fluorescent overlap was quantified by Imaris as voxels (i.e., overlap between volumetric pixels in either channel).

    Techniques: Derivative Assay, Membrane, Labeling, Isolation, Immunofluorescence, Concentration Assay